الفهرس 
Title : Correlation between Interleukin-6 and epithelial-mesenchymal transition markers in breast cancer patients with positive and negative lymph node
AbstractOnly 14 pages are availabe for public view
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Abstract
Metastasis is the most studied process in cancer biology including breast cancer. Development of metastases depends on multiple factors that determine overall tumor cell growth, survival, angiogenesis, and invasion. For epithelial malignancies, epithelial- mesenchymal transition (EMT) is considered to be the crucial event in the metastatic process. Interleukin-6 (IL-6) is one of the cytokines that have been known to correlate with disease stage and progression in breast cancer. The aim of the present study was to determine the role of secretions of tumor-associated leukocytes (TALs) isolated from negative and positive lymph nodes (nLNs and pLNs) respectively, breast cancer patients in regulating EMT mechanism and the associated signaling pathways. Furthermore, we assessed the levels of IL-6, p-NF-mB/p65 (Ser276) and infiltration of CD45+ in carcinoma tissues and lymph nodes of nLNs and pLNs breast cancer patients and investigate their role in the epithelial-mesenchymal conversion, namely expression of the epithelial marker E-cadherin and the mesenchymal marker vimentin. Our results revealed that IL-6, vimentin and p-NF-mB/p65 (Ser276) were highly expressed in breast carcinoma tissue and lymph nodes of pLNs than those with nLNs breast cancer patients. On contrary, E-cadherin was downregulated in breast carcinoma tissues of pLNs compared with nLNs breast cancer patients. This observation was reversed in lymph nodes, whereby E-cadherin was upregulated in lymph node tissues of pLNs com-pared with those of nLNs breast cancer patients