الفهرس 
Phytochemical and biological study of oroxylum indicum L. cultivated in Egypt
ContentsOnly 14 pages are availabe for public view
 |  | from | 250 |  |  |
| | | from | 250 | | |
Abstract
Phytochemical and biological study of Oroxylum indicum L. belonging to Family bignoniaceae was carried out. Review of literature about different constituents of the plant , as well as, different reported biological activities was performed. Phytochemical screening of different plant parts was carried out. Analysis of volatile oil of plant leaves was performed by GC/MS. Preparation of ethanol (70%) extract of the leaves and its different fractions was done. chromatographic investigation of petroleum ether fraction of the leaves led to isolation and identification of Ý-sitosterol acetate, 6-methoxy apigenin (hispidulin) and stigmasterol for the first time from the leaves. Investigation of phenolic content of ethyl acetate fraction of the leaves led to isolation and identification of acteoside, apigenin , luteolin and methyl gallate for the first time from the leaves. Total phenolic and flavonoid content was estimated colorimetrically. Also, HPLC was used for determination of phenolic content. Investigation of saponin content of n-butanol fraction led to isolation and identification of oleanolic 3-O-glucopyranosyl for the first time from the leaves. Also, estimation of saponin content by haemolytic index method was performed. Biological study was conducted in vitro including antimicrobial study by agar well diffusion method, cytotoxic activity by SRB method against three different human cell lines: (breast carcinoma cell line) MCF7, (liver carcinoma cell line) HEPG2 and (cervix carcinoma cell line) HELA and antioxidant activity was screened by DPPH assay (2, 2-diphenyl-1-picrylhydrazyl). Also, in vivo biological study included determination of LD₅₀, antioxidant activity which was estimated by determination of blood glutathione level in the blood of alloxan-induced diabetic rats and hepatoprotective activity by CCl4-induced hepatotoxicity