الفهرس 
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Materials and MethodsOnly 14 pages are availabe for public view
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Abstract
The analysis was based on collecting about 110 diseased and freshly dead broiler chickens (aged from 1 to 45 days old) and 65 dead in shell chicken embryos.
1-The clinical signs and PM lesions of the naturally infected birds were described and discussed.
2-Bacteriological examination was conducted in order to isolate Enterococcus bacteria.
• Then the positive colonies on KF streptococcal agar were cultured onto bile esculine azide agar and 6.5% NaCl broth and incubated at 37°C for 24 hours. The results were as follows:-
• the percent of isolated Enterococcus spp. from bile esculine test & 6.5% NaCl broth culture represent about (61.8%) in liver,spleen & heart, (57.3% )in intestine, (10% ) in joint,( 8.2%) in brain and 34 from 65 samples(52%) dead in shell chicken embryos.
• Enterococcus isolates were also cultured on MacConky agar No.2 and Sheep blood agar and incubated at 37°C for 24 hours.
• All the Enterococcus isolates were having the ability to grow at 10 and 45°C and the biochemical reactions revealed that the isolated organism was catalase-negative and positive for gelatin hydrolysis and the organism was non- motile, positive to starch hydrolysis and litmus milk test,no pigment production and no hemolysis.
3- Classification of Enterococcus isolates according to biochemical tests as follow:
• E. hirae was isolated from liver, spleen and heart (66.2%), intestine (66.7%), joint ( 81.8%),brain (66.7%) and dead in shell chicken embryos (44.1%).
• E. durans was isolated from liver, spleen and heart (22%), intestine (14.2%), joint (18.1%), brain (33.3%) and dead in shell embryos (41.2%).
• E.fecalis was isolated from liver, spleen and heart (11.8%),intestine (19%) and dead in shell chicken embryos (14.7%) and did not isolated from joint or brain.
4- The pathogenicity test:
• E.hirae isolate and 4-day-old cobb broiler chicks were used in this experiment.
• Clinical signs,pm lesions,deaths and mortality rates were reported and discussed.
• In an attempt to re-isolate E.hirae from the inoculated chicks and contact chicks, the organism was reisolated from internal organs and biochemically tested.
5- Antimicrobial sensitivity test:
E.hirae isolates were resistance to oxytetracycline, co-trimoxazole, enrofloxacin, oxacillin, tetracycline, erythromycin and doxycycline, also sensitive to ampicillin and neomycin, intermediate to amoxicillin and penicillin g.