الفهرس 
introductionOnly 14 pages are availabe for public view
 |  | from | 53 |  |  |
| | | from | 53 | | |
Abstract
Pseudomonas aeruginosa (PA) is a Gram-negative pathogen responsible for
fatal nosocomial infections worldwide. Iron is essential for Gram-negative
bacteria to establish an infection. Therefore, iron acquisition proteins (IAPs)
of bacteria are attractive vaccine targets. A “Reverse Vaccinology” approach
was employed in the current study. Expression levels of 37 IAPs in various
types of PA infections were analyzed in seven previously published studies.
The IAP vaccine candidate was selected based on multiple criteria, including
a high level of expression, high antigenicity, solubility, and conservation
among PA strains, utilizing suitable bioinformatics analysis tools. The
selected IAP candidate was recombinantly expressed in Escherichia coli and
purified using metal affinity chromatography. It was further evaluated in vivo
for protection efficacy. The novel immune adjuvant, naloxone (NAL),