الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
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Abstract
“An eco-friendly multi-analyte high -performance thin layer chromatographic densitometric determination of amoxicillin, metronidazole, and famotidine in their ternary mixtures and simulated gastric fluid: a promising protocol for eradicating Helicobacter pylori”
Gastrointestinal tract disorders constitute a heavy burden to healthcare providers. To eradicate Helicobacter pylori infection, different triple therapy protocols have been proposed. Among which are combinations of proton pump inhibitors (e.g., omeprazole), histamine-2 receptor antagonists (e.g., famotidine), along with antibiotics (e.g., amoxicillin). In this work, a sensitive and accurate high performance thin- layer liquid chromatographic method was developed for the simultaneous determination of amoxicillin, metronidazole, and famotidine in bulk powder and laboratory-prepared combined-tablet mixtures. Complete separation of the cited compounds was achieved using pre-coated silica gel plates with a mixture of methanol: chloroform: toluene: water: glacial acetic acid (5:2:1.5:0.5:0.1v/v/v/v/v) as the mobile phase. The method was fully validated as per the international conference of harmonization guidelines. Good linearity, a correlation coefficient of 0.9991, was obtained in the concentration range 0.1-1.6 μg/band (amoxicillin), 0.1-0.9 μg/band (metronidazole) and 0.1-0.9 μg/band (famotidine). Since the method allowed the determination of the three compounds in combined tablets with a high degree of selectivity, accuracy, precision, with cost effectiveness, it could be used for regular quality control. Moreover, the applicability of the.