الفهرس 
Antihepatotoxic role of melatonin, ursodeoxycholic acid and Balanites aegyptiaca extract against methotrexate induced liver toxicity in rats
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Abstract
Purpose: Methotrexate (MTX) is extensively used in the treatment of a variety of cancerous as well as inflammatory and auto-immune diseases. However, its use is restricted by its rigorous draw-backs primarily on the liver. This study aimed to compare the degree of ameliorative effects of melatonin, ursodeoxycholic acid and Balanites aegyptiaca against hepatotoxicity induced by MTX for one month. Materials and Methods: Eighty adult male rats weighing (180-200gm) were randomly divided into eight equal groups: Control, MTX (13.4 mg/kg b.wt.), MEL (10 mg/kg b.wt.), BA (100 mg/kg b.wt.), UDCA (20 mg/kg b.wt.), MTX+MEL, MTX+BA and MTX+UDCA. Results: Administration of MTX showed significant increase in liver function biomarker tests, oxidative stress parameters as well as TNF-Ü level. Whereas total protein, albumin, TAC, GSH, GPx, GR, GST, SOD, CAT and Hb levels were significantly decreased in MTX treated group. In addition, there was a significant increase in kidney functions tests in MTX group. Histopathological examination and immunohistochemistry supported the biochemical results. Both MEL and BA were able to normalize the levels of the biochemical and oxidative stress parameters whereas; no improvement was noticed in UDCA treated group. Conclusion: BA may be as promising as MEL in the hepato-protection against MTX toxicity through their antioxidant and radical scavenging activities. On the other hand, it is not recommended to co-administer UDCA with MTX as it enhanced inflammation and damage to the liver