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Abstract
Four vaccines formula with different adjuvants (liquid paraffin oil (vaccine no. 1), MontanideTM ISA 71 (vaccine no. 2), MontanideTM ISA 201(vaccine no. 3) and MontanideTM Gel01 (vaccine no. 4)) and four vaccines formula by using Date palm (Phoenix dactylifera L) and Mentha piperita immunomodulators included in liquid paraffin oil adjuvant infectious coryza vaccine only in ratio of 1mg or 2mg of each immunomodulators, these 8 vaccines were used in vaccination of 8- 10 weeks old chickens and evaluated by using Microplate agglutination test (MAT), ELISA and challenge test. It was cleared that, vaccines no. 3, 4 and 2 yielded a maximum of significant (P < 0.05 ) higher and faster rate of antibody titer estimated by MAT and ELISA with a higher challenge protection percentage than vaccine no. 1. Evaluation of the humoral immune response of chicken vaccinated with liquid paraffin oil adjuvanated infectious coryza vaccine with 1 mg ( vaccine no. 5) and 2mg ( vaccine no. 6) of Date palm and also with 1mg ( vaccine no. 7 ) and 2mg ( vaccine no. 8) of Mentha piperta by using MAT, ELISA, cleared that antibody titer increase significantly (P < 0.05 ) at relative rates against the three antigens (W, Modesto, 0222) of Av. paragallinarum reaching maximum rates at the 10th to 12th week post immunization also the use of these immunomodulators give a higher protection percentage than vaccine no. 1. The data also cleared that the antibody titer was higher by using of 2mg of either Date palm or Mentha piperita included in the vaccine than using of 1mg. The results obtained in this study concluded that, the infectious coryza vaccine adjuvanted with either MontanideTM ISA71VG, MontanideTM ISA 201VG or MontanideTM Gel 01 adjuvant yielded immune response in chicken better than that adjuvanated with liquid paraffin oil. Also using an immunomodulators as Date palm and Mentha piperita included in liquid paraffin oil adjuvant infectious coryza vaccine can enhance immune response of chicken against infectious coryza disease