الفهرس 
Resistance and virulence genes of E.coli in broiler chickens
ABSTRACTOnly 14 pages are availabe for public view
 |  | from | 188 |  |  |
| | | from | 188 | | |
Abstract
A total of 120 broiler chickens (76 diseased living and 44 freshly dead), 3-5 weeks old were collected from different localities in Egypt ( Giza, Kaliobyia, Monofyia, Dakahlyia, Sharkyia, Kafr El-sheikh and Behyira ).The internal organs ” heart, liver, bone marrow, spleen and kidney” were collected from the examined broilers. The percentage recovery rate of E. coli from broiler chickens was (18.33%). The antimicrobial sensitivity test for the recovered isolates against 10 antibiotic agents revealed that, resistance to Doxycycline was (100%), Amoxicillin (90.91%), Ciprofloxacin (86.37%), Gentamicin (81.82), Sulfamethoxazole/Trimethoprim (77.27%), Chloramphenicol (72.73%), Danofloxacin (68.18%), Nitrofurantoin (68.18%), cefotaxime (40.91%) and Colistin sulphate (27.27%) respectively. The molecular characterization of some ESÝLs genes (blaTEM, blaCTX, blaCMY1, blaSHV and blaOXA1) of E. coli using the Polymerase Chain Reaction (PCR) revealed that blaTEM, blaCTX and blaSHV were detected in the plasmid at a percentage of 81.82% , while all ESÝLs genes could not be detected in the chromosomal DNA. The molecular characterization of some virulence genes (iroN, ompT, hlyF, iss and iutA) revealed that all these genes detected in both chromosomal DNA and plasmid at percentages of 77.27% and 59.09% respectively