الفهرس 
Screening of bacterial contamination during gelatin production process
AbstractOnly 14 pages are availabe for public view
 |  | from | 163 |  |  |
| | | from | 163 | | |
Abstract
This study aims to detect the contamination extent during gelatin manufacturing, with comparing the in vitro efficacy of commonly used antibiotics and essential oils to reduce microbial contamination. In addition, isolate and purify gelatinase enzyme from the active microbial contamination. Bacterial strains were isolated from different stages of gelatin manufacturing process, identification and growth optimization of the strains was done according to conventional microbiological methods. Gelatinase was screened using gelatin agar plate method. Antibiotic sensitivity and antibacterial activity of essential oils were done by the disk diffusion method. GC/MS technique was used to analyze essential oils constituents. Isolation and purification of gelatinase was done by ammonium sulphate fractionation and dialysis followed gel filtration using Sephadex G-150 column and DEAE-Cellulose column. SDS-PAGE and zymography were performed to reveal molecular size of gelatinase. Bacillus genera were the predominant contamination over bacterial isolates. Majority of the isolates have moderate gelatinase activity. Gatifloxacin of the fluoroquinolones was the most effective antibiotic against bacterial isolates. Cinnamon oil exhibits the highest antibacterial activity with the highest sensitivity rate (97.6%) and low MIC value (0.195ol/ml). GC/MS analysis of effective essential oils showed that monoterpenes were the predominant amongst other chemical constituents. Main constituent of cinnamon oil was cinnamaldehyde (63.69%). SDS-PAGE analysis showed that gelatinase appeared as single band on the gel with molecular size ” " ~ " ”100 KDa. Zymography of the gelatinase showed that it appears as clear zone against darker background at almost the same molecular size (” " ~ " ”100 KDa)