الفهرس 
Title : Detection of dna from semen stains submitted to different washing conditions
ContentsOnly 14 pages are availabe for public view
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Abstract
Background: The ability to extract DNA from semen stain is a key element in forensic genetics. Attempts to remove semen stain using different laundering conditions represent a routinely faced forensic problem. Objective: This work was done to evaluate the effect of different laundering conditions on the quantity of extracted DNA from semen stained cloth. Method: In the current study, Semen samples were applied to cotton and polyester cloth. After drying, the cloth was submitted to different laundering conditions (laundering by water at 0C0, at 70C0 temperature and detergent). DNA extraction was done to estimate the quantity of extracted DNA by spectrophotometer. Results: It is observed that DNA still could be detected in cotton and polyester after washing in water at 0C0 temperature, in water at 70C0 temperature and in detergent group. There is highly statistical significant decrease (P {u2264} 0.001) in the amount of extracted DNA in all group samples (after washing) compared to control group (before washing).Washing by detergent proved to have the most destructive effect on the DNA stains on cloths followed by washing by water at 70C0 while washing by water at 0C0 temperature showed the highest recovery of DNA in cloth The amount of the DNA detected in cotton samples before washing was higher than the amount detected in polyester samples before washing. Conclusions: DNA in semen stain cloth could be recovered after exposure to different laundering conditions