الفهرس 
Title : Study on the influence of the greater cairo metropolitan area on the microbial diversity and bacterial genotypic determinants in the Nile River Water
ContentsOnly 14 pages are availabe for public view
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Abstract
Fresh water systems need microbial community analysis to monitor the influence of xenobiotic and anthropogenic inputs particularly in urban and industrial settings. As the Nile river is the primary and main water source for Egypt, it is essential to study and monitor how the microbial community in and around the Nile{u2019}s dynamic setting is evolving. Surface water samples and sediments from the Nile river banks were collected in both summer and winter from five different locations that span the natural flow of the river crossing the Cairo metropolis passing by both residential and industrial areas. Next generation sequencing followed by appropriate computational analysis were used to analyze the 16S rRNA gene composition. Conventional microbial count was also carried out. Results showed that in spite of the wide physicochemical variation and the river{u2019}s water level differences encountered in summer and winter, there was a striking stability in the Nile microbiome community structure along the examined geographical urban sites and between the wet and dry seasons as evidenced by the high proportion of shared operational taxonomic unit (OTUs) values among all samples. Cultures based methods revealed significant differences in the measured bacterial load in water samples between the different locations in both seasons. Analysis of community structure revealed that Proteobacteria was the most abundant phylum in both water and sediment followed by Acidobacteria and Chloroflexi in the sediment, and by Cyanobacteria, Actinobacteria and Bacteroidetes in the water. Analysis of potential biomarkers in water communities revealed that the difference between summer and winter samples was mainly under the effect of phylum Cyanobacteria. Functional predicted metabolic pathways for the samples analyzed revealed that despite the different pollutants encountered in the Nile ecosystem, rare of them are implicated as pathogenic pollutants