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العنوان
Studies on the genetic improvement of jojoba plant in Egypt and Sudan /
الناشر
Marwa Mokhtar Abdalgaleel Khair Allah ,
المؤلف
Marwa Mokhtar Abdalgaleel Khair Allah
هيئة الاعداد
باحث / Marwa Mokhtar Abdalgaleel Khair Allah
مشرف / Atef Zakaria Sarhan
مشرف / Amira Shawky Soliman
مشرف / Ahmad Othman Hamada
تاريخ النشر
2019
عدد الصفحات
166 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
5/9/2019
مكان الإجازة
جامعة القاهرة - كلية الدراسات الإفريقية العليا - Natural Resources
الفهرس
Only 14 pages are availabe for public view

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from 187

Abstract

This study was carried out at the Natural Resources Department, Faculty of African Postgraduate Studies, Cairo University, Giza, Egypt. And Fruit, Ornamental plants, Woody Trees Department, Horticulture Research Institute, Giza, Egypt; during the successive seasons of 2015-2018. The aim of this work is to improve micropropagation conditions of Simmondsia chinensis using micro-cuttings in order to explore the possibility of inducing mutation through in vitro technique and the production of salinity tolerant mutants. Sterilized jojoba micro-cuttings were cultured on {u00BE} MS starting medium free of growth regulators. Regenerated shoots were exposed to 0.0, 10, 15, 20 and 25 Kr doses of gamma ray. Or treated with 0.0, 1%, 2%, 3% and 4% concentrations of EMS. Multiplication MS medium contained different concentrations of BA alone or with Kin. Afterwards, to evaluate the reaction of the resulting mutagenized tissues to salinity stress; Regenerated shoots were treated with sea salt concentrations at 0.0, 15%, 25%, 50% and 100.0%. Results indicated that the highest number of shoots (12 shoot/ explant) was obtained using 3.0 g/l BA in medium. While, combination of 3.0 mg/l BA and 0.5 mg/l Kin resulted in high shoot formation and number of shoots of (9.50 shoot/ explant). Results showed that jojoba shoots irradiated with 20 kr gamma ray and treated with 25% and 50% sea salt resulted in survival percentage of (80% and 60%) respectively in comparison with control (93.33%)