الفهرس 
Title : Role of lipopolysaccharide in gene expression alteration and immune responsein isolated goat monocytes
AbstractOnly 14 pages are availabe for public view
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Abstract
Lipopolysaccharide was extracted from E-coli O157:H7 and blood samples (8-10 ml) collected in heparinized sterile screw-capped tube from the jugular vein of twenty Egyptian goats were used in this study to be treated with Lipopolysaccharide (LPS) which is an endotoxin used as a model of inflammation. LPS offers an attractive model for inducing inflammation due to its ability to provoke secretion of tumor necrotic factor TNF-Ü and multiple cytokines (e.g. IL-1Ý, IL-6, and IL-10) so LPS is an important model used as an immune stimulant. The importance of endotoxin is based on a variety of biological responses which this molecule provokes both in vitro and in vivo. The biologically active moiety of endotoxin, the lipopolysaccharide (LPS) has been identified and biochemically characterized for a variety of enteric organisms. Further, the toxic principle of LPS lipid A has been defined and synthesized. Genes in LPS-stimulated monocytes is evaluated by quantitative real-time PCR using beta-actin for normalization. Our results shownfour genes (TNF-Ü, IL1-Ý, IL6 and IL10) were quantified by RT-qPCR in LPS-stimulated goat monocytes in vitro and viability of monocyte cells treated with LPS were evaluated using MTT assay.The result showed decrease in cell viability with increasing LPS concentrations in contrast to control group. RT-qPCR results showed an increase in gene expression of IL1-Ý, IL-6 and TNF-Ü but no change for IL10. Conclusion: LPS incubated with monocyte with different concentration resulted in decreased cell viability and increase gene expression