الفهرس 
Title
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Abstract
Two salt stress related genes namely, Glyoxalase II (Gly II) and salt overly sensitive (SOS2) were isolated by RT-PCR from an Egyptian Sorghum bicolor cultivar.The RT-PCR amplified bands for the SbGly II and SbSOS2 genes were 1011 and 1350 bp in size, encoding polypeptide chain of 336 and 449 amino acids with deduced molecular mass 37.0 and 50.3 kDa, respectively. The nucleotide sequences of the SbGly II and SbSOS2 genes were BLASTn searched at NCBI to explore the homologous genes. The sequence comparison of both genes (SbGly II and SbSOS2) with the published sequences in NCBI database revealed that the SbSOS2 gene is a unique gene since it showed an amino acid sequence homology ranging from 99-84% with the corresponding sequences in the database. While, the Egyptian SbGly II gene revealed 100% homology with the published S. bicolor. The full length of the SbGly II and SbSOS2 genes were submitted to the GeneBank database with accession number KP883296 and KY202762, respectively. The phylogenetic analysis for SbGly II and SbSOS2 from Egyptian sorghum showed a high similarity with Gly II and SOS2 from Zea mays. While they were distant from those of Oryza sativa. Both genes were cloned individually into the pYES2 expression vector for expression in Saccharomyces cerevisia