الفهرس 
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Abstract
Duchenne Muscular Dystrophy (DMD) is one of the most common severe form of childhood muscular dystrophy affecting 1:5000 newborn males. The lack of functional dystrophin results in repeated cycles of muscle necrosis and regeneration leading to eventual replacement of muscle fibers by adipose and connective tissue. Aim of the study is to establish measurements of the levels of circulating serum miR-1, miR-206, miR-133, MM9-9, TIMP-1, PON1, ARE, TC, TG, LDL, and HDL in a group of DMD steroid naïve patients, and DMD patients who took the steroids, and investigate whether such biomarkers can have diagnostic advantage for disease detection. Fourteen Blood samples were collected on EDTA, from steroid treated pediatric patients between the ages of 5 and 13 years old, fifteen blood samples were collected from recently diagnosed DMD patients between the ages of 4 and 7, fifteen blood samples were collected from healthy controls between the ages of 4 and 13, blood samples were centrifuged; eppendorf tubes of the serum were stored at -80 degrees, for further tests to be carried on them. Serum levels of the previously mentioned biomarkers were assayed and statistical tests were carried out, results showed significance difference of the assayed biomarkers concentration between the three test groups, which imply that such biomarkers’ concentration is reliable as a diagnostic tool for the early discovery of DMD.