الفهرس 
Vitamin DOnly 14 pages are availabe for public view
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Abstract
Background: Vitamin D is not only involved in calcium and bone metabolism, but also in the regulation of proliferation and differentiation of many cells, in addition to its immunoregulatory, anti-angiogenetic, and antioxidant properties. An association was found between vitamin D deficiency and increased risk of Metabolic Syndrome components such as abdominal obesity with vitamin D deficiency and Vitamin D Receptor (VDR) gene polymorphisms contributing to its development. Increased risk of obesity, metabolic disturbances and bone mass disturbances among certain VDR alleles has been proven. The importance of VDR in the etiopathology of obesity is associated with the occurrence of polymorphisms FokI, BsmI, ApaI, TaqI. VDR expression in adipocytes plays a role in the regulation of energy metabolism and the induction of obesity.
Objective: The aim of this study is to determine the association between VDR gene polymorphism namely BsmI in Egyptian obese male medical students and vitamin D status.
Patients and Methods: This study based on a large project named (Nutritional Assessment of Medical Educational Students (NAMES) conducted in 2018 with a total number of 700 healthy participants (males and females). Out of the seven hundred participants a total of fifty healthy male subject met our inclusion criteria and were enrolled in the study. They were attending Ain Shams university hospital in the period between April 2019 and November 2020. The laboratory work was conducted at the Clinical Pathology Department, Faculty of Medicine, Ain Shams University Hospital. The subjects were subdivided into two groups; group I: included twelve obese male subjects, with non – deficient vitamin D level (> 20 mg/dl), group II: this group included eighteen obese male subjects, with deficient vitamin D level (<20 ng/dl). VDR SNP (BsmI) rs 1544410 analysis was performed by a commercial real time PCR kit pre-designed TaqMan probes. The DNA needed for the PCR was purified by spin procedure (QIA amp DNA Mini Kit).
Results: No statistically significant relation between vitamin D level and all InBody bioelectrical impedance, as well as between the BsmI Gene Polymorphism and all InBody bioelectrical impedance parameters, (P value > 0.05). No statistically significant relation between group I and group II with Bb genotype and BB genotype. Our study revealed that prevalence of BB genotype more in the vitamin D deficient candidates and Bb genotype carriers were more frequent among obese participants than BB genotype which showed the prevalence of the “b” allele more among the obese participants. However, the results did not reach a significant level. No statistically significant relation between iron profile (iron level, ferritin level, TIBC, transferrin saturation) and BsmI Gene Polymorphism. (P value > 0.05). While the relation between HbA1c and BsmI Gene Polymorphism was statistically significant (P Value =0.002) with an increase in mean value in Bb genotype than BB genotype. There is also a statistically significant difference between BB Genotype and Bb Genotype regarding eosinophils (P value 0.045) with increase in mean value in Bb Genotype than BB genotype. Other parameters of the complete blood count (CBC) show no statistically significant relation with Bsml Gene Polymorphism. (P value > 0.05).
Conclusion: Our study results showed no significant relation between the VDR gene polymorphism (BsmI) with BMI nor the different InBody bioelectrical impedance with the prevalence of BB genotype among the vitamin D deficient obese students and the frequency of ‘b ‘allele among obese candidate . Moreover, no association was observed between the BsmI gene polymorphism and vitamin D Levels. We observed a correlation between BsmI gene polymorphism and HbA1c and eosinophilic count which need further studies to reveal more about this associations.