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Abstract This study was planned to isolate the fungi from samples of wastewater contaminated with azo dyes, and in vitro utilization of the isolated fungi in bioremediation of two azo dyes. The obtained results were as follows: - One hundred and twenty fungal isolates belonging to 28 species and 7 genera have been isolated in this study from twenty wastewater samples collected from six cities namely Assiut city (Assiut Governorate), the 10th Ramadan city (El-Sharqia Governorate), El-Mahalla Al-Kubra city (ElGharbia Governorate), Kafr El-Dawar city (El-Behira Governorate), Alexandria city (Alexandria Governorate) and Port Said city (Port Said Governorate). - Aspergillus represented by 14 species was the most prevalent genus obtained from all samples comprising 58.21 % of total fungi. The bluecolored samples gained the highest number (8) of Aspergillus species, followed by brown-colored, red-colored and the colorless samples (5 each), and the black ones (3). - Aspergillus flavus was the most common species constituting 46.15 % of total Aspergillus and 26.86 % of total fungi, followed by Aspergillus terreus constituting 20.51 % of total Aspergillus and 11.94 % of total fungi. - Aspergillus parasiticus and A. niger accounted for 8.97 % and 7.69 % of all Aspergillus, and 5.22 % and 4.48 % of total fungi, respectively. - Penicillium represented by 6 species was the runner of Aspergillus obtained from all samples and comprised 13.43 % of total fungi. |