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Abstract This experiment was carried out at the Animal Production Experimental farm, Faculty of Agriculture, New Valley University, Egypt. The study was started from May 2019 tell September 2020 in New Valley governorate during summer season. A total number of twelve healthy Aberdeen Angus cows about 7-8 years of age and an average body weight of 460-520 kg and ten healthy Aberdeen Angus bulls average body weight of 210-220 kg were used in this study. The animals were divided randomly in to two equal groups, viz, control group and betainesupplemented group, with six cows and five bulls in each group. All cows were fed on basal diet which was formulated according to NRC, (2000) for beef cattle. The basal diet consists of 60% concentrate mixture and 40% wheat straw. Animals of the control group received no supplementation , while the supplemented group received 30g /betaine/ day for the entire period of the study in addition to the regular maintenance diet. The objective of the present study was to evaluate the ability of betaine to overcome the harmful effects of heat stress and its effect on productive and reproductive performance of Aberdeen Angus cattle under New Valley hot climatic conditions. and compile evidence that betaine supplementation may be an effective strategy to minimize the impact of heat stress in cows. |