الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 140 |  |  |
| | | from | 140 | | |
Abstract
Abstract
The present study aimed to compare the efficacy of laser, PRP (platelet-rich plasma) or PRP/laser combination in promoting the healing of experimentally induced full thickness skin defect in dogs. In this study, three male adult mongrel dogs were subjected to 4 full thickness skin defects (circular full thickness skin wounds: 3 cm in diameter: 2 at left side of thoracic region and 2 at the right side) each received a specific treatment. Hence, 4 groups were evaluated: control group (skin defect was created caudally at the left side of thoracic region and treated with conventional treatment); PRP group (skin defect was created cranially at left side of thoracic region and treated with single subcutaneous infiltration of inactivated autologous PRP); Laser group (skin defect was created caudally at the right side of thoracic region and treated with laser application for one session) and PRP/laser group (skin defect was created cranially at the right side of thoracic region and treated with single subcutaneous infiltration of autologous PRP in conjunction with laser application for one session). In all wound groups, the measured variables were clinical (determination of the percentage of wound size and contraction), histopathological, biochemical (activity of catalase enzyme and concentration of malondialdehyde), molecular (expression of vascular endothelial growth factor and collagen I alpha 2 genes) and immunohistochemistry (tumor necrosis factor-α and nuclear factor-kappa Beta) evaluations.
The results revealed that, based on clinical evaluation, greater reduction in the percentage of wound size and greater elevation in percentage of wound contraction were detected in PRP, laser and PRP/laser groups compared to control group with the greatest changes being detected in PRP group at most time points, better histopathological findings were demonstrated in PRP and PRP/laser groups compared to other groups. At one time point, the area percent of tumor necrosis factor-α was significantly lower in PRP and PRP/laser groups compared to other groups while the area percent of nuclear factor-kappa Beta was significantly lower in laser group compared to other groups. Catalase enzyme activity was elevated in laser, PRP and PRP/laser groups (the greatest elevation was in PRP group) compared to control group. Reduced concentration of malondialdehyde and greater expression of vascular endothelial growth factor and collagen I alpha 2 genes were more notable in PRP and PRP/laser groups compared to other groups (greater changes were detected in PRP relative to PRP/laser at some time points).
Based on the obtained results, laser, PRP and PRP/laser were found to be effective in accelerating healing of acute full thickness skin wounds in dogs with a greater efficacy for PRP and PRP/laser. PRP and PRP/laser seemed effective in improving the quality of healing. Changes in the measured biochemical, molecular and immunohistochemistry variables were helpful in determining some of the mechanisms behind the effect of laser, PRP and PRP/laser combination on healing of acute full thickness skin wounds in dogs. On conclusion, PRP and PRP/laser could be feasible and effective therapies for management of acute full thickness skin wounds in dogs.