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العنوان
Molecular Typing of Salmonella Species Isolated from Processed Meat A Thesis
Presented /
المؤلف
Farghaly, Abdallah Ahmed Shehata.
هيئة الاعداد
باحث / عبد الله احمد شحاته فرغلي
مشرف / عبد العظيم محمد الجمال
مناقش / فوزي رياض محمد الصعيدي
مناقش / محمود عزت السيد
الموضوع
Salmonella. Meat.
تاريخ النشر
2022.
عدد الصفحات
107 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
15/5/2022
مكان الإجازة
جامعة قناة السويس - كلية الطب البيطري - البكتريولوجيا والمناعه والفطريات
الفهرس
Only 14 pages are availabe for public view

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Abstract

This study was conducted to investigate the prevalence of Salmonella species in
processed meat as well as molecular typing and antibiogram of the recovered
Salmonella isolates. A total of 200 processed meat samples (Sausage, burger, Kofta
and luncheon) (50 of each) were randomly collected from different supermarkets and
butchers in Suez Governorate, Egypt. The bacteriological examination was carried out,
where the prevalence of Salmonella species was 18% (9/50), 10% (5/50), 6% (3/50)
and 4% (2/50) for sausage, burger, kofta and luncheon, respectively. S. Typhimurium
and S. Enteritidis were the most prevalent with a percentage of 5% and 2.5%,
respectively in processed meat samples. In addition, the antimicrobial susceptibility
testing was carried out. The PCR was used for detection of virulence genes in the
isolated Salmonella serotypes, where all the tested serotypes were positive for invA and
stn gene. While 84.2% harbored the hilA gene and 21% harbored the pefA gene.
Moreover, the prevalence of strA gene was 47.4%. In conclusion, S. Typhimurium was
the most prevalent Salmonella spp. in processed meat. The most prevalent virulence
genes in Salmonella were invA and stn gene. While strA gene is the most prevalent
antimicrobial resistance gene in Salmonella spp. isolated from processed meat
products.