الفهرس | Only 14 pages are availabe for public view |
Abstract Ten wild species of lamiaceae, Stachys aegyptiaca, Teucrium polium, Lavandula pubescens, Rosmarinus officinalis, Ballota kaiseri, Mentha longifolia, Salvia multicaulis, Phlomis aurea, Origanum syriacum subsp. Sinaicum, Thymus decussatus were collected from Sant Catrine peninsula at April 2019 and identified then examined physiologically and anatomically. Results revealed that essential oil of Thymus decussatus had high efficiency as an antimicrobial agent on all tested pathogens especially with Staphylococcus aureus and Candida albicans with maximum clear zone of microbes (53 and 45mm, respectively) under laboratory conditions compared to other species, due to its high essential oil content of terpenes as thymol (5.11%), manool oxide (0.13%), solvanol (0.36%), isopropyl palmitate (0.27%), heyderiol (0.34%) as well as high value of total antioxidants (inhibited 77.6 % of 2,2-diphenyl-1-picrylhydrazyl). Species were differed in leaf and stem histology, types of trichomes and stomata, concentration of pigments, reducing sugars, free amino acids, soluble protein, phenolics and essential oil. Shoot tips of Mentha longifolia produced the maximum number of shoots/explant (17), number of leaves/ explant (11), maximum value of stem length (7.54 cm) after application of 1ppm of benzyl amino purine (BAP) without naphthalene acetic acid. Constituents of essential oil were differed between in vitro propagated Menta and its wild plants but showed the same effect as antimicrobial agent. It can recommend that, using of wild Thymus plant as highly antipathogenic agents and possibility of in vitro propagation of Menta using BAP at 1ppm. |