الفهرس 
AcknowledgementsOnly 14 pages are availabe for public view
 |  | from | 182 |  |  |
| | | from | 182 | | |
Abstract
Diabetic wound infection is the most prevalent complication in the world. Hyperglycemia, occurs due to lack of insulin secretion from the β cells in the pancreas (type 1) or the insulin does not work as a result of imbalance in the ability of insulin receptors on the cells (type 2) or both reasons. Experimentally type 1 diabetes can be induced by STZ that destroy β cells, leading to hyperglycemia and hpo-insulinemia. Diabetes causes dysfunction in the immune system and the rate of secretion of cytokines, which increases the rate of apoptosis and fibrosis, and delays wound healing. The immune suppression lead to the aggressive bacterial colonization, which in turn may lead to gangrene and limb amputation.
The present study was aimed to evaluate the modulating role of fig latex on the pathogenic bacteria isolated from diabetic foot wounds (in vitro study). Additionally, to evaluate the effect of topical application of the fig latex on type 1diabetes mice model (in vivo study) induced by STZ and its efficacy to accelerate skin wound healing.
II- In vitro experiment: 50 swab samples were recovered from 50 patients with diabetic foot wound infection. The swab sample were cultured to different culture media for bacterial isolation, purification and identification. The microscopic examination was used to identify the bacterial shape and arrangement and their susceptibility towards Gram stain. We recovered and identified 100 bacterial isolates; the biofilm assay was performed to detect the ability of these isolated pathogenic bacteria to form biofilm.