الفهرس 
Pathological studies on the protective effect of purslane (portulaca oleracea) seeds extract on acrylamide induced toxicity in rats
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Abstract
Acrylamide (ACR) is a chemical that naturally forms in starchy food products during high-temperature cooking, including frying, baking, roasting and also industrial processing, in this study we aimed to evaluate the toxic effect of ACR in nervous and reproductive systems of male rats through molecular, biochemical, pathological and immunohistochemical examinations. Also to evaluate the protective effects of portulaca oleracea seed hydroethanolic extract (PSE) on acrylamide -induced toxicity. PSE was subjected to phytochemical investigation using ultra-high-performance liquid chromatography (UPLC) coupled with quantitative time of flight mass spectrometry (qTOF-MS).A number of 42 male Wister albino rats were divided into six groups each group has 7 rats including negative control, ACR-intoxicated group (10 mg/kg/day), PSE treated groups (200 and 400 mg/kg/day), and ACR + PSE treated groups (200 and 400 mg/kg/day, respectively). All treatments were given intragastrically for 60 days. The ACR treated group showed reduced relative organ body weight, while cotreatment with PSE showed significant increase of the weight. ACR caused a significant decrease in plasma total antioxidant capacity (TAC) and glutathione (GSH) activity, and an increase malondialdehyde (MDA) levels in testes and brain compared to the control group. In contrast, PSE treatment significantly increased the (TAC) and (GSH) activities, and deceased MDA levels. PSE markedly ameliorated brain damage as evidenced by the decreased lactate dehydrogenase (LDH), increased acetylcholinesterase (AchE) activities, as well as the increased brain-derived neurotrophic factor (BDNF) that were altered by the toxic dose of ACR