الفهرس 
Title : Diagnostic accuracy of salivary DNA integrity index in oral malignant and premalignant lesions
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Abstract
Aim: This study aimed to identify the accuracy of salivary DNA integrity index in differentiating between oral premalignant lesions and oral cancer. Methodology: This phase II diagnostic test accuracy study included 93 patients divided into three groups: 30 Oral cancer patients, 33 patients with oral premalignant lesions and 30 normal individuals who acted as controls. Oral rinse was collected from all participants and they all underwent conventional visual and tactile examination, and patients with oral lesions had the diagnosis confirmed by histopathological examination of tissue biopsy. DNA integrity index was determined as the ratio between ALU247 and ALU115 measured by qPCR. Results: There was no statistically significant difference regarding ALU115, ALU247 and DNA integrity index (DII) between the three studied groups. The DII was significantly higher in the oral cancer group than the oral lichen planus cases, when considered alone, while no significant vii difference was found between the oral cancer and the leukoplakia cases.There was no correlation between DII and lesion size. Also DII did not differ between groups regarding tumor grade nor degree of dysplasia The DII sensitivity, specificity, positive and negative predictive values were 73%, 45%, 55% and 65% respectively.Conclusion: Salivary DNA integrity index showed poor diagnostic abilities in differentiating between the oral cancer and premalignant lesions groups. Additionally, salivary cell free DNA has questionable stability, which makes this test impractical for clinical use