الفهرس 
AcknowledgmentsOnly 14 pages are availabe for public view
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Abstract
Flurbiprofen (FLUR) is a potent non-steroidal anti-inflammatory drug used for the
management of arthritis. Unfortunately, its therapeutic effect is limited by its rapid
clearance from the joints following intra-articular injection. To improve its therapeutic
efficacy, hyaluronic acid-coated bovine serum albumin nanoparticles (HA-BSA NPs) were
formulated and loaded with FLUR to achieve active drug targeting. NPs were prepared by
modified nano-emulsification technique and their HA coating was proven via turbidimetric
assay. Physicochemical characterization of the selected HA-BSA NPs revealed entrapment
efficiency of 90.12 ± 1.06%, particle size of 257.12 ± 2.54 nm, PDI of 0.25 ± 0.01, and
zeta potential of -48 ± 3 mv. The selected formulation showed in-vitro extended-release
profile up to 6 days. In-vivo studies on adjuvant-induced arthritis rat model exhibited a
significant reduction in joint swelling after intra-articular administration of FLUR-loaded
HA-BSA NPs. Additionally, there was a significant reduction in CRP level in blood as well
as TNF-α, and IL-6 levels in serum and joint tissues. Immunohistochemical study indicated
a significant decrease in INOS level in joint tissues. Histopathological analysis confirmed
the safety of FLUR-loaded HA-BSA NPs. Thus, our results revealed that FLUR loaded
HA-BSA NPs have a promising therapeutic effect in the management of arthritis.