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Abstract Background: Microbial keratitis is a serious ocular infectious disease and one of the preventable leading causes of blindness worldwide, more predominantly in the developing countries. It affects both sexes, and all ages. The incidence, risk factors, causative organisms, and fate vary from region to region and according to different management plans, still corneal culture is the gold standard procedure and it is recommended to be performed in all cases of microbial keratitis. Purpose: To identify the incidence, risk factors, bacterial/fungal spectrum causing the ulcers and to determine the best possible empirical therapy and specific therapy of infective keratitis at Cairo University hospitals. Methods: A cross sectional analytic study on 85 cases of infective keratitis, carried out at Cairo University Hospitals, Kasr Al Aini, over a period of 9 months from September 2020 to May 2021. History aspects including the systemic and local risk factors, clinical features of the ulcer which were noted by slit lamp examination at the time of presentation. Corneal scrapings were performed and underwent direct microscopy and culturing in appropriate media using standard laboratory protocols |