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العنوان
Effect of gamma radiation on bacterial redox protein azurin /
الناشر
Khaled Mohamed Elsaied ,
المؤلف
Khaled Mohamed Elsaied
هيئة الاعداد
باحث / Khaled Mohamed Elsaied
مشرف / Doria Aly Zaki
مشرف / Zahira Sayed Tawfik
مشرف / Mourad M.S. Abulezz
تاريخ النشر
2021
عدد الصفحات
150 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
24/10/2021
مكان الإجازة
جامعة القاهرة - كلية العلوم - Botany
الفهرس
Only 14 pages are availabe for public view

from 185

from 185

Abstract

The D10 value of a clinical isolate of P. aeruginosa was calculated to be 0.12 kGy. The total protein-N contents of untreated and irradiated cells with {uF067}-irradiation up to 1.2 kGy declared a noticeable increase in the amount of protein contents in cells treated with 0.8 kGy (V1).The D10 value of (V1) was estimated to be 0.14 kGy. By repeating exposure of (V1) to Y-irradiation at 0.8 kGy (V2), (V3), (V4), (V5) and (V6) were induced. The maximum value of protein-N contents was estimated in (V3). The protein profile from SDS-PAGE analysis indicated that the maximum percentage of azurin was recorded in (V3). The amino acid profile and functional groups of purified azurin from (V3) and the standard azurin revealing the similarity between the extracted and the standard azurin