الفهرس 
Electrospun nanofibers technology
Electrospun nanofibrous composite preparationOnly 14 pages are availabe for public view
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Abstract
Innovative two diverse electrospun nanofibrous composites for wound dressing and tissue engineering applications based on natural therapy berberine chloride hydrate (BER) and antimicrobial drug metronidazole (MTZ) not only facing microbial invasion but also enhancing wound healing along with lowering antimicrobial resistance. Herein, we report and investigate the electrospinning of the blended Poly- 3-hydroxybutyrate (PHB) and gelatin (Gel) nanofibrous composites (PHB/Gel). The as prepared electrospun nanofibrous composites (PHB/Gel) with different ratios (8:2, 7:3 and 6:4) and the as prepared electrospun nanofibrous composites (PHB/Gel) with different ratios loaded with at two different concentrations of the two different drugs (E1 and E2) and (Z1 and Z2) respectively, utilizing a highly volatile solvent 2, 2, 2- triflouroethanol (TFE) were investigated. Chemical and morphological characterizations, mechanical properties, swelling, porosity, hydrophilicity, thermal stability, antimicrobial activity and cytocompatibility of the as prepared crosslinked electrospun nanofibrous composites were evaluated. Furthermore, the release profiles of the as prepared electrospun nanofibrous composites loaded with BER and MTZ implies sustained drug release profiles. Moreover, in vivo wound healing was evaluated in wistar rats for 14 days and the experiments revealed that the as prepared electrospun nanofibrous composite loaded with either BER oe MTZ augments the transforming growth factor (TGF-β) signaling pathway, reduces the pro-inflammatory cytokine (IL-6), and those meritoriously enhanced wound healing in experimental mice. As a result, the as prepared electrospun nanofibrous composite PHB/Gel loaded with BER and MTZ are considered to be a prospective candidates for wound dressings and tissue engineering applications, specifically for the healing of long-term wounds and infections.