الفهرس 
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Abstract
• Alpha methyl scopolamine was administered subcutaneously at 1 mg / kg as an antimuscarinic drug 30 minutes before pilocarpine to prevent its marginal cholinergic side effects; salivation, dacriorrea, stroke, bradycardia and hypotension. It does not cross the BEE and does not interfere with the focal impacts of pilocarpine on seizure recruitment. 3 Collection treated with Pilocarpine + Diazepam; in which rodents received a solitary infusion of diazepam as a kind of prospective anticonvulsant drug at a dose of 1 mg / kg, ip, one hour before inclusion in pilocarpine epilepsy as in group 2. 4 Pilocarpine + Collection treated with larginine; in which the rodents received a solitary infusion of Larginine as nitric oxide (NO) before a dose of 500 mg / kg, ip, one hour before enrollment in pilocarpine epilepsy as in pack 2. 5 Collection treated with pilocarpine + aminoguanidine; in which the rodents received a solitary infusion of aminoguanidine; a specific inducible NO synthase inhibitor (iNOS) at a dose of 200 mg / kg i.p. one hour before pilocarpine acceptance of epilepsy as in group 2. 6 Pilocarpine + Verapamil, calcium channel blocker (CCB); collection processed; in which rodents were given a single serving of 20 mg ̷ kg verapamil one hour before acceptance of the epilepsy pilocarpine as in group 2. All tests were conducted in a quiet laboratory with bright conditions constant between 10 a.m. and 3 p.m. After the pilocarpine infusion, the rodents were placed in individual enclosures, monitored closely and continuously for one hour and as often as possible thereafter for 24 hours when the test was completed. During this period, rodents were checked for support: A. The score depended on the Racine scale, as illustrated above (Racine et al. 1972) with the support phases: stage (0) ; no irregularities, stage (1); Developments of the mouth and face, stage (2); Gesticulate the head, stage (3); Clonus of the forelimbs, stage (4); Reproduction, stage (5); Raise up and fall.Complete motor seizure, with transient loss of postural control, is called stage 5 motor seizure. B. Seizure rate (rodents with predominantly clonic attacks of the forelimbs were considered safe): [No. of rodents with seizure / no. rodents per group] x 100. C. Time of onset of next pilocarpine attack; the period of inactivity. D. Percentage of mortality after one and 24 hours. Blood tests were taken from the retro-orbital venous plexus either after the first seizure or towards the end of prime time for rodents that did not exhibit seizures. Blood was pooled, centrifuged, and sera collected and stored at 20 ° C for the safety of S100B protein, TNFα, and malondialdehyde (MDA). Towards the end of the entire exploration period (24 hours), the durable rodents were lost by cervical separation.The confiscated and dead rodent heads were quickly analyzed and the spirits tenderly taken out for the planning of brain homogenization. Arrangement of brain homogenates for biochemical measurement: minds washed with typical saline solution to remove blood tests and mental tissue from the hippocampus and transient flapPilocarpine injection induces astrocytes hyperactivity as evidenced by increased S100B protein, the marker of BBB damage, multiple strong immunoreactive enlarged astrocytes with obvious ramified cytoplasmic processes were seen with immuno-histological study if compared to that of the control group. In particular, gliosis and scarring changes were eliminated in animals treated with diazepam and appeared similar to that of the control group. In L-arginine - treated group, S100B antibody-reactive free astrocytes and that sheath the blood vessels were markedly increased if compared to that of the control group. In aminoguanidine treated rats, free immune-reactive astrocytes appeared to be reduced and moderately stained if compared to that surround the blood vessels. Verapamil-treated group showed fewer S100B immunoreactive astrocytes that stained lighter if compared to Pilocarpine-treated group and all abnormal morphological changes were decreased.