الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Carbapenem antibiotics are important therapeutic agents in the health care settings, they are frequently used as an empiric therapy for life-threatening infections as well as infections with multi-drug-resistant Gram-negative bacilli. Carbapenemase-producing Carbapenem-resistant Enterobacteriaceae (CRE) are significant public health challenge worldwide.
Enzymatic resistance to carbapenems in Gram-negative bacteria is mainly due to two types of β-lactamases. The first group of enzymes uses serine as the active site amino acid to inactivate c rb penems, nd the m in inhibitors of the serine β-lactamases are tazobactam, clavulanic acid and sulbactam. The second group of enzymes uses a zinc ion, hence their name, the metallo-β l ct m ses (MBLs). The ion chelator ethylene diamine tetra-acetic acid (EDTA) is a common inhibitor of MBLs.
This study aimed to evaluate and compare the diagnostic performance of the phenotypic methods in relation to the molecular methods for detection of carbapenamases-producing genes. The studied genes included the NDM; one of the MBLs and the blaOXA-48; one of the serine β l ct m se in E. coli and Klebsiella species which were isolated from different departments and units in Menoufia university hospitals.
This study was conducted in Menoufia University Hospitals during the period from April 2020 to March 2021. It included 65 carbapenem-resistant and 35 carbapenem-sensitive Enterobacteriaceae strains which were isolated from 350 patients who were admitted to the Menoufia University Hospitals. An