![]() | Only 14 pages are availabe for public view |
Abstract Summary 56 Summary SLE is an autoimmune disease that affects people all over the world. The pathogenic processes of SLE are the production of autoantibodies and the deposition of antibody-containing immune complexes in blood vessels, which result in a variety of clinical manifestations and target tissue damage. SLE is distinguished by the involvement of complex genetic and environmental components. The genetic component is important in the etiology of SLE disease with multiple genetic variants as single nucleotide polymorphisms (SNPs) contributing to the disease’s onset. Single nucleotide polymorphisms (SNPs) in the area of the Tumor necrosis factor superfamily number 4 (TNFSF4) gene have been linked to a variety of chronic systemic inflammatory disorders, including SLE. This study aimed to detect the association of TNFSF4 (rs1234315) gene polymorphism and susceptibility of systemic lupus erythematosus. 100 subjects were included in this study and classified into two groups; group 1 included 50 patients with SLE and group 2 included 50 age and gender-matched subjects as the control group. All individuals were subjected to full history taking and full clinical examination. Systemic lupus erythematosus disease activity index (SLEDAI) was assessed in all the cases included in the study. Different laboratory parameters were done for all the individuals in the study including CBC, ESR, C-reactive protein, liver function tests, kidney function tests, Protein/Creatinine ratio, urine microscopic examination, ANA, C3, C4, and anti-dsDNA. Genotyping for TNFSF4 gene polymorphism was done by allelic discrimination assay of real time PCR. The results of this study revealed the following: ● The studied groups were age and gender matched ● The mean SLEDAI score of SLE cases was 14.14 ± 4.35 with a range between 5 and 35. ● CBC, ESR, CRP, C4, ANA, and anti-ds DNA were found with high significance in SLE cases. ● SLE was associated with the affection of the kidney functions as revealed by elevation in serum creatinine, urea, protein/creatinine ratio, which was significantly higher in the SLE cases with no statistical significance according to liver investigations. ● The wild type (C/T)of TNFSF4 gene was significant in the control group while the mutant type (T/T) was highly significant in SLE cases. ● T allele of TNFSF4 gene was highly significant in the SLE cases. |