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Abstract As the number of populations grow, the need for water (both household and agricultural) increases. At the same time, there is a water supply problem. Wastewater treatment and reuse for irrigation might become one of the most important options for reducing the problem of water scarcity. Yeast industry is one of the most important industries that used as substrate in some food processing industries. This industry produces large quantities of wastewater that characterized with high organic impurities due to the use of molasses which is a sugar manufacturing by-product. Several treatment methods such as activated sludge, moving bed biofilm reactor (MBBR), electrocoagulation, and chemical treatment could be used for yeast industry wastewater treatment. In this study, the treatment was done on four samples of yeast wastewater using a scale-pilot consists of 3 tanks with dimension (50*30*30 cm). The first tank work as a primary sedimentation tank, the second was the aeration tank with air diffusers and the third tank was final sedimentation, the 3 tanks followed with sand filter. The 3 tanks were installed on different levels to feed each tank under gravity. Four trails were performed as following: the activated sludge as a control attempt, integrated fixed biofilm reactor FBR using different media, gravel only in the second trial, zeolite only in the third trial, and combined between zeolite and gravel in the fourth trial. Results show that the most efficient trail was the fourth trail, FBR with combined media gravel and zeolite with a HRT 24 h where the removal efficiency of COD, BOD, TSS, TKN, and TP was 94.5%, 96.3%, 97%, 85%, and 83.5%, respectively and the effluents met the acceptable limitation of law 48 year 1982 for reuse for irrigation purposes. |