الفهرس 
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Abstract
Ulcerative colitis is a chronic gastrointestinal inflammatory disease caused by a dysregulated
immune response against different environmental triggers in genetically predisposed
individuals. The role of internal micro-environment is outstanding in this regard where UC
patients have consistently been shown to have less diverse microbiome compared to healthy
controls along with shift towards pathogens and away from healthy microbiota composition.
Targeting the gut microbiome through fecal microbiota transplantation has been appealing
option for treatment of patients with UC based on the previous positive experience of such
therapy in C. difficile. However, lack of standardization of the process is a major hurdle before
approval of FMT for UC. selection of a donor with a potential positive outcome is an everwanted
goal in the pursuit to an optimum FMT process.
This open-label randomized study was conducted on 20 patients (10 males, 10 females) with
active UC who received FMT from 10 healthy unrelated donors via either colonic or nasogastric
routes with an aim of identifying the ideal donor associated with the best response in respective
recipients. Patients with a Mayo score 5 – 10 (median 8), aged 22 -63 years old (Mean ± SD 44
± 10.50), were randomized to either colonic (n = 12, 60%) or nasogastric (n = 8, 40%) FMT
after matching for age, sex & disease activity. Both patient groups received 8 doses of FMT
over 8 weeks. Donors were recruited after passing the FMT donor health questionnaire and
donor screening program. Donors were 8 males (80%) and 2 females (20%) aged 22- 48 years
old (Mean 33.6 ± 9.57), BMI range 23.51 – 30.04 kg/m2 (Mean 24.76 ± 2.93).
For the purpose of the study, donors were assessed for their usual dietary intake using the EPIC
food frequency questionnaire in addition to assessment of their gut microbiota composition to
identify the diet pattern and gut microbiota composition associated with response in UC
patients. Diet data was evaluated using the EPIC FETA programme. Fecal samples from donors
were stored at –80º c before DNA extraction, PCR, and sequencing by next generation
sequencing methods. Microbiota raw data was submitted for analysis using the Qiime Package