الفهرس 
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Abstract
The present study was conducted to assess the meat products bacterial contamination of B. cereus in Port Said Governate, 140 random samples of meat products including sausage, sliced pastrami, minced meat, luncheon, beef meat, liver, and salami (20 for each) were collected from various retailers and supermarkets for bacteriological examination for detection the prevalence of enterotoxigenic B. cereus strains in these samples, beside the phenotypic characterization and detection of some virulence and anti-microbial resistance genes in them. Bacteriological examination of the collected samples resulted in, (48) samples (34.2%) positive reaction to B. cereus groups which include (32) B. cereus (22.8%), (5/25%) from sausage samples; (1/5%) sliced pastrami; (3/15%) minced meat; (11/55%) luncheon; (8/40%) beef meat ;(4/20%) liver and (0/0%) from salami. 7 B. mycoids (5%) and 9 B. thuringiensis (6.4%) and (11) samples (7.8 %)
positive reaction to B. subtilis. The isolated B. cereus strains were enterotoxigenic ones, as they had
haemolytic and Lecithinase activities. The PCR results cleared that, the diagnostic, phylogenetic marker gene of B. cereus groEL (533pb) was amplified in all 7 studied B. cereus isolates, cytK (421pb); pc-plc (411pb); nhe (766pb); ces (1271pb) and hblD (148pb) enterotoxigenic virulence genes were detected in 2; 2; 4;1 and 0, respectively, out of 7 studied isolates. Meanwhile bla (680 pb) and tetA
(502 pb) anti-microbial resistance genes were >detected in 2 and 0 respectively, out of 7 studied
isolates. Moreover, the groEL gene in isolated
B. cereus sequences was seem to be identical 99.79%
identity with the reference strains reported on Gene
Bank. The obtained nucleotide sequences were
submitted in the Gene Bank with accession number
(MZ424866). The isotated B. cereus showed
resistance to Penicillins (Oxacillin) and Polymyxin
(polymyxin B). While 96.9% of the isolates were
resistant to Penicillins (Amoxicillin and Penicillin),
87.5% to Cephalosporins (Cefepime) ,75% to
Ansamycin (Rifamycin), 62.5 % to Cephalosporins
(Cefaclor and Cephradine) and 60.8% to
Cephalosporins(Ceftriaxone ). However, the isolated
B. cereus isolates were sensitive to Fluoroquinolones
(Levofloxacin), 93.8% to Phenicol