الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Staphylococcusaureus is a versatile bacterium with multiple antibiotic resistance and there is no successful vaccine was launched against it till this moment. The objective of this study is offering the potential vaccine candidates against S. aureus infection based on reverse vaccinology approach. Ten genes candidates selected based on in silico studies were screened by PCR in 75 different S. aureus isolates. The gene which was detected in most of the clinical isolates was chosen for cloning in pH6HTN His6HaloTag® vector and expressed in E. coli BL21 (DE3), purified by immobilized metal affinity chromatography (IMAC). Finally, its in vitro reactivity with S. aureushuman serum antibodies was tested. After PCR screening,phosphatidylinositol phosphodiesterase (plc) and zinc metalloproteinase aureolysin (aur) were detected in 100% and 98% of all S. aureus isolates, respectively. aur was successfullycloned and expressed using 0.5 mM IPTG for 4 hours at 37°C that was the optimum condition of Aur expression. His-tag Aur protein was then detected at ” ” ” ” " ~ " ” ” ” ” 86 KDa as single band after western blot assay.Aur protein could be used as a baseline for downstream application to develop a potential vaccine against S. aureus infection.