الفهرس 
Histological structure and development of skeletal musclesOnly 14 pages are availabe for public view
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Abstract
The potential role of mesenchymal stem cells derived exosomes on the healing of grade II experimentally induced skeletal muscle injury in adult male albino rats. Histological study.
Background: Musculoskeletal injuries are the most common cause of severe long-term pain and physical disability. However, there is still no efficient treatment that leads to prevention of muscle fibrosis. Cell-Free therapy using mesenchymal stem cells (MSCs) derived exosomes could provide a new promising therapy.
Aim: To study the potential role of MSCs-derived exosomes on the healing of experimentally induced grade II skeletal muscle injury in adult male albino rats.
Materials and Methods: Forty male albino Wister rats were used in this study. Five out of them were used for isolation of exosomes. The rest were randomly divided into four groups. group I (Sham group), group II (untreated muscle injury group), group III (muscle injury treated with exosomes) and group IV (muscle injury treated with saline). Rats from group I, II and III were subdivided into two subgroups; subgroup “a and b” in which rats were sacrificed 10 days and 20 days after muscle injury, respectively. group IV was sacrificed 20 days after muscle injury. Muscle specimens were collected and prepared for H&E and Masson`s trichrome stain. Immuno-histochemical staining for detection of collagen type I and developmental myosin (MHCd) were also done. Morphometric study and statistical analysis were performed.
Results: Fibrous tissue and inflammatory cellular infiltration with significant increase in the mean area percentage of collagen fibers were noticed in untreated muscle injury subgroups compared to the corresponding exosomes treated subgroups. In comparison to untreated muscle injury group II, exosomes treated group III showed noticeable improvement and healing of muscle injury and significant increase in immune-histochemical reaction of developmental myosin. group IV showed no significant structural differences as compared with group II.
Conclusions: It was concluded that the treatment of experimentally induced grade II muscle injury with exosomes was effective in promoting muscle healing and reducing muscle fibrosis.