الفهرس 
Acknowledgmen
IntroductionOnly 14 pages are availabe for public view
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Abstract
Cancer constitutes one of the most serious causes of death worldwide. CRC, in particular, is the 4th commonest cancer in the world, and it is the 7th commonest cancer in Egypt. In addition, colon cancer is the second-most dangerous type of cancer, affecting both men and women. Therefore, scientists aim to increase the effectiveness of the existing treatments, eliminate cancer side effects and to improve as much as possible the quality of life of patients. The application of EMFs in the treatment of different kinds of cancer whether alone or in combination with other treatments seems to be a promising therapy that has drawn attention during the last decades. In this context, the current study aimed to investigate the potential beneficial effects of ELF-EMF application alone and in combination with broccoli extract in the treatment and protection from colon cancer in an animal model. For the purpose of this study, 42 male Albino rats (Rattus rattus) were randomly divided into 7 groups (6 rats/group), as follows: G1 was control group, G2 was exposed to ELF-EMFs (50 Hz, 0.9 m T) 75 mins, daily for 4 weeks. G3 was injected intraperitoneally with PHZ (90mg/Kg) only twice a week for 4 weeks, G4 was injected intraperitoneally with PHZ (90mg/Kg) twice a week for 4 weeks, and followed by ELF-EMFs (50 Hz, 0.9 mT) 75 mins daily, for another 4 weeks. G5 was injected intraperitoneally with PHZ (90mg/Kg) twice a week for 4 weeks, and followed by broccoli extract administration (300 mg/kg) twice/week regularly for 4 weeks. G6 was injected intraperitoneally with PHZ (90mg/Kg) twice a week for 4 weeks, and followed by ELF-EMFs (50 Hz, 0.9 mT) 75 mins daily plus broccoli extract administration (300 mg/kg) twice/week regularly for 4 weeks. And lastly, G7 was for experience the protective effect of both ELF-EMFs and broccoli extract in which rats were injected intraperitoneally with PHZ (90mg/Kg) twice a week and co-exposed to ELF-EMFs (50 Hz, 0.9 mT) 75 mins daily plus broccoli extract administration (300 mg/kg) twice/week regularly for 4 weeks. The results revealed that: PHZ injection was able to induce carcinogenicity in the intestinal tissues of rats. Treatment with ELF-EMFs (50 Hz, 0.9 mT) 75 mins daily for 4 weeks slightly reduced and ameliorated the PHZ induced intestinal carcinogenicity which noticed in a marked improvement in histological architecture of the crypt with minor size abnormalities. Also, there were improvement in Summary 79 immunohistochemical observations showed in p53 protein up regulation and survivin protein down regulation .That results were agreed with physiological parameters which revealed a marked enhancement compared to PHZ-injected rats .However, treatment with broccoli extract in LD50 of( 300 mg/kg) had a minor amelioration noticed histologically in the majority of intestinal crypts which still had disorganized architecture with extensive nucleus divisions and numerous mitotic figures .And immunohistochemically by the expression of p53 protein which was reduced and survivin protein which was strongly expressed .physiological parameters showed also a significant abnormalities compared to control group. Interestingly, the usage a combination of ELF EMFs (50 Hz, 0.9 mT) daily for 75 mins daily and broccoli extract(300 mg/kg) twice weekly, both were applied in parallel for 4 weeks ,as a treatment in (G6) or as a protection in (G7) resulted in prominent reduction and amelioration indicating in the PHZ-induced intestinal carcinogenicity. That amelioration was shown in histological, immunohistochemical and physiological results which were approximately similar to control especially in protection group.