الفهرس 
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Abstract
The present study was conducted on two species of 300 freshwater fishes. (160 Oreochromis niloticus and 140 Clarias gariepinus)that were randomly collected in different seasons from different localities in Ismailia governorate from December 2018 to November2019. The examined fishes revealed few pathognomonic signs. Heavy infested O. niloticus revealed paleness of the skin,loss of condition and dullness.While infected Cl.gariepinus showed hemorrhagic ulcers, detached tail fins, injuries on the skin and hemorrhage on the ventral surface of the fish. Postmortem examination of O.niloticus showed yellowish discoloration of the liver. While in Cl.gariepinus,the kidneys showed slight enlargement and yellowish cysts embeded in them and these cysts were also observed embeded in muscles.The total infestation rate was(74.33%).It was(73.13%) in O.niloticus and(75.71%)in Cl.gariepinus.Helminthes isolated from O.niloticus were digenetic trematodes Eumesenia aegypticus and Acanthocentis tilapiae isolated from intestine of O.niloticus.Cl.gariepinus infested with Orientocredium batrachoides (digenetic trematodes),Monobothrioides species, Polyonchobthorium clarias(Cestodes),Procamallanus laeviconchus and Paracamallanus cyathopharynx(Nematodes)which were isolated from intestine . Encysted metacercariae were found in different internal organs of both species as gills,spleen,heart and musculature.The research revealed that the highest prevalence was in autumn and winter.Male fish was proved to be of higher prevalence than female among both species. The highest prevalence among both species were EMC which might be transmitted to man through eating raw fish.Molecular diagnosis(PCR)was used to confirm infestation with helminthes parasites.