الفهرس 
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Abstract
Rheumatoid arthritis (RA) is a common inflammatory and angiogenic
disease. While the importance of inflammation in RA is well understood, little is
known about the molecular mechanisms promoting angiogenesis in RA.
The oxygen-sensitive transcription factor, which allows for adaptation of
hypoxia to joints environment in rheumatoid arthritis is hypoxia-inducible factor 1
alpha (HIF-1α).
The aim of this work is to study the role of HIF- 1α gene polymorphisms
(rs12434438) in patients with rheumatoid arthritis and its relation to disease
activity in Egyptian population.
This study was conducted on 80 subjects, their ages ranged between 33-65
years. They were divided into 2groups
Group(I): (40) Patients with rheumatoid arthritis who met the criteria
established by the American College of Rheumatology (ACR) for RA.
group (II): (40) subjects divided in to two groups: Control group (IIa)
(20) Patients with osteoarthritis and Control group (IIb) (20) normal subjects as
control group with matched age and gender with patient’s groups.
All participants included in the study were subjected to: full history taking,
clinical examination, routine laboratory investigations including: liver and kidney
function tests, Complete Blood Picture, Erythrocyte Sedimentation Rate, CReactive
Protein, Anti-CCP, Rheumatoid Factor, and (HIF- 1α) (rs12434438)
single nucleotide polymorphism analysis by real time PCR.
Regarding our results: CBC, liver and kidney functions were statistically
insignificant but RF, CRP, ESR and Anti-CCP were statistically significant in RA
group.
Genotype distribution of HIF-1α: AA genotype and A allele were
statistically significant higher in RA group than in control group. GG, AG
genotype and G allele were statistically significant higher in both control normal
and OA group than in RA group.
The univariate analysis revealed that age, albumin, urea, RF and GG
genotype are independent risk factor for RA, but in multivariate analysis, only RF
is dependent risk factor for RA.