الفهرس | Only 14 pages are availabe for public view |
Abstract Rheumatoid arthritis (RA) is a common inflammatory and angiogenic disease. While the importance of inflammation in RA is well understood, little is known about the molecular mechanisms promoting angiogenesis in RA. The oxygen-sensitive transcription factor, which allows for adaptation of hypoxia to joints environment in rheumatoid arthritis is hypoxia-inducible factor 1 alpha (HIF-1α). The aim of this work is to study the role of HIF- 1α gene polymorphisms (rs12434438) in patients with rheumatoid arthritis and its relation to disease activity in Egyptian population. This study was conducted on 80 subjects, their ages ranged between 33-65 years. They were divided into 2groups Group(I): (40) Patients with rheumatoid arthritis who met the criteria established by the American College of Rheumatology (ACR) for RA. group (II): (40) subjects divided in to two groups: Control group (IIa) (20) Patients with osteoarthritis and Control group (IIb) (20) normal subjects as control group with matched age and gender with patient’s groups. All participants included in the study were subjected to: full history taking, clinical examination, routine laboratory investigations including: liver and kidney function tests, Complete Blood Picture, Erythrocyte Sedimentation Rate, CReactive Protein, Anti-CCP, Rheumatoid Factor, and (HIF- 1α) (rs12434438) single nucleotide polymorphism analysis by real time PCR. Regarding our results: CBC, liver and kidney functions were statistically insignificant but RF, CRP, ESR and Anti-CCP were statistically significant in RA group. Genotype distribution of HIF-1α: AA genotype and A allele were statistically significant higher in RA group than in control group. GG, AG genotype and G allele were statistically significant higher in both control normal and OA group than in RA group. The univariate analysis revealed that age, albumin, urea, RF and GG genotype are independent risk factor for RA, but in multivariate analysis, only RF is dependent risk factor for RA. |