الفهرس 
English CoverOnly 14 pages are availabe for public view
 |  | from | 119 |  |  |
| | | from | 119 | | |
Abstract
Introduction: chronic hyperglycemia is a critical condition which leads to different complications in various region of the body including oral cavity. Oral complications related to diabetes mellitus (DM) include xerostomia, tooth decay, oral candidiasis, burning mouth, periodontal disease, and gingivitis, periapical lesion, geographic and fissured tongue. Degree of those complications always related to degree and duration of hyperglycemia. Aim of the study: the present study analyzed the effect of 808nm low power diode laser and metformin on human periodontal ligament stem cells (HPDLSCs) when culture in high glucose media. Material and methods: HPDLSCs isolated from freshly extracted impacted third molar teeth then characterized and divided into eight groups as a follow ; group 1, normal control. group 2, diabetic ; group 3, irradiation with 1 J/cm2;group 4, culture with metformin and irradiation with 1 J/cm2 ;group 5, irradiation with 2J/cm2;group 6, culture with metformin and irradiation with 2J/cm2;group 7, irradiation with 3 J/cm2 and group 8, culture with metformin and irradiation with 3 J/cm2 . Extra 5 ٪ glucose level was added to diabetic groups 24 hours before laser irradiations, while 10 nmol of metformin was added to metformin groups according to study design. Irradiation done with diode laser 808 nm 24 and 48 hours. Cell viability was analyzed with MTT assay 24 hours post -irradiation. Real-time (PCR) used to evaluate gene expression of (Nrf2, Keap1, PIK3, and HO-1). ELISA reader used to evaluate proteolytic activity of the biomarkers (ROS, TNF-α, IL-10) within the cells 48 hrs. Post-irradiation. . Results: regarding to our results, photobiomodulation at 1, 2, 3 J/cm2 in combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of Nrf2, Keap1, PIK3, and HO-1 levels (p< 0.05). Conclusion: Under the condition and limitations of the present study. Photobiomodulation with 3 J/cm2 combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.