الفهرس 
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Abstract
The aim of this work is to carry out phytochemical and biological study of Cuscuta campestris belonging to family Convolvulaceae. In this study, eight compounds were isolated and identified using various spectroscopic techniques from which two compounds are isolated for the first time from Cuscuta campestris. Also, the extracts were screened for their cytotoxic activities. Part I: Phytochemical investigation of Cuscuta campestris Yunck. The air-dried and powdered of whole plant of Cuscuta campestris family Convolvulaceae was extracted by maceration with methanol then concentrated to a syrupy consistency which was then fractionated successively with petroleum ether, methylene chloride, ethyl acetate, and n-butanol. The eight isolated compounds (β –sitosterol, stigmsterol, (1S, 2S)-1, 2, 3-Trihydroxy-1-(3’, 4’-methylenedioxyphenyl) propane, episesamine, oleanolic acid, linocinnamarin, mixture of quercetin-3-O-β-D-glucoside and quercetin-3-O-β-D-galactoside, and astragallin) were identified using various spectroscopic techniques including; UV, IR, 1D and 2D-NMR, and MS. Part II : Biological study of different extracts from Cuscuta campestris Yunck. This study screened the cytotoxic activity on six cancer cell lines. These cell lines are human lung cancer (A-549), human colorectal (cecum) cancer (LS-513), human cervical cancer (HeLa), human ovarian cancer (SKOV-3), human bladder cancer (T-24) and human pancreatic cancer (PANC-1) by using SRB assay. These activities can be partly attributed to methylene chloride fraction which showed the highest in vitro inhibitory activities among the petroleum ether and ethyl acetate fractions on all types of tested cell lines. While ethyl acetate fraction is cytotoxic on human panceriatic cancer, while the petroleum ether fraction showed cytotoxic effect on human panceriatic, ovarian and lung cancer.