الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
In order to investigate the prevealnce, the antibiogram and the molecular typing of virulence genes (eaeA, stx1, stx2, f41, lt, sta) and antibiotic resistance genes (aadB, sul1, blaTEM) in E.coli incriminated in calf diarrhea, a total of 274 fecal samples were collected from diarrheic calves in Damietta and Elsharkia Governorates, Egypt. The collected samples were subjected to bacteriological exam, where the prevelance of E.coli was 28.8% (n=79). Serotyping of the isolated E.coli strains revealed (7) serogroups (O128, O111, O26, O125, O91, O45 and O119 as well as untypable strains. The antmicrobial susceptibility was carried out, where the isolated strains were resistant to were more frequently resistant to amoxicillin/ clavulanic acid, ampicillin / sulbactam, trimethoprim-sulfamethoxazole and streptomycin and sensitive to enrofloxacin, gentamicin, ceftiofur and amikacin. PCR was used for detection of virulence genes and antibiotic resistance genes in the recoverd strains. The tested strains were found to harbor eaeA, stx1, stx2, f41, lt, sta, aadB, sul1 and blaTEM genes. In conclousion, the routine application of antimicrobial susceptibility testing is necessary due to the emergence of multi-drug resistant E.coli strains. The combination of both phenotypic and genotypic characterization is a reliable epidemiological tool used in identify of E.coli incriminated in diarrhea.