الفهرس 
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Abstract
Brucellosis is a worldwide zoonotic disease, now considered endemic in most parts of Egypt. The study aimed to spot light upon the public health significance and zoonotic importance of Brucellosis in New Valley Governorate and to investigate the seroprevalence of brucellosis in human and livestock in two region in New valley governorate from December 2018 to February 2020. A total of 1254 animals (673 cattle, 348 sheep and 233 goat) and 523 human serum samples were examined for brucellosis using RBT and thereafter-select random samples (15 from cattle, 7 from sheep, 3 from goat and 45 from human) subjected again to complement fixation test (CFT) and Enzyme linked immune sorbent assays test (ELISA)for comparison and to detect the sensitivity and specificity of RBT. The prevalence according to RBT, was (0%) in cattle, sheep and goat while it was (23.9%) in human. Concerning human, there was higher percentage of infection in EL Kharga (33.6%) than recorded in ELDakhla (7.7%) and higher percentage of infection in the age group >40 years (28.57%) than in the age group <40 years (17.20 %), Also, males showed higher percentage (26.11%) than females (22.5%) with no significant difference. Considering the human occupation, abattoir workers was the most infected (33.3%), followed by farmers (31.25%) and animal keeper (20.6%), the lowest prevalence was observed in the house wives (18.8 %). There was a significant correlation at (P <0.05) between different age groups, locality, season, contact with animals, occupational groups and prevalence of human brucellosis in New Valley governorate. Conclusively, brucellosis represents an alarming problem among residents in the New Valley governorate, so many measures to reduce the prevalence among animal population and human in the study region may include restriction of marketing raw milk and enhancement of the public health awareness.