الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Hypertension is a multifactorial and polygenic disorder where several susceptible genes interact with the environmental factors, demographic and genetic factors Objective: This study aims to check for the association of genetic polymorphisms of endothelial nitric oxide synthase (eNOS, E298D) and cytochrome P450 2J2 (CYP 2J2 -50G/T), CYP2D6 *1, *3, *4, and *5 variants with hypertensive subjects.Subjects and methods: Participants included 123 hypertensive cases in addition to 429 unrelated healthy control subjects. For all participants, DNA was extracted from peripheral lymphocytes and analyzed using light cycler PCR technique for the characterization of genotypic and allelic variants of eNOS E298D, CYP2J2 -50 G/T and CYP2D6*1, CYP2D6*3, CYP2D6*4, and CYP2D6*5 genes.Results: Hypertensive cases showed higher frequency of mutant eNOS heterozygous ED genotype compared to controls, yet statically insignificant (27.6% vs. 21.1%, OR =1.5, 95%CI =0.9-2.8, p=0.1). Hypertensive cases also showed higher frequency of mutant CYP2J2 -50 GT genotypes compared to controls that was also statistically insignificant (13.8% vs. 10.7%, OR =1.3, 95%CI =0.7-2.4, p=0.4). All other cases subgroups including complicated cases with cardiac, diabetic and renal disorders showed non significant difference compared to controls regarding the studied eNOS and CYP2J2 gene polymorphisms.Comparing cases of hypertension and controls as regard the genotypic allelic variants of CYP2D6 gene polymorphisms showed significantly higher wild genotype 1/1 among cases compared to controls (85.4% vs. 74.8%, p=0.01) with a lower frequency of mutant genotypes 4/4 (1.6% vs. 8.6%, p0.008). This phenomena were manifest among cases subgroups with obesity that had significantly lower mutant homozygous forms than obese controls (2.3% vs. 9.5%, p=0.04) and cases with cardiac complications (88.2% vs. 74.8%, p=0.01).