الفهرس 
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Abstract
A total of 100 random samples of fresh chicken cuts (breast, thigh) (50 of each) were collected from different butcher’s shops at El- Menoufia governorates. These samples were examined for coliforms counts, isolation and identification of coliforms strains and identification of E.coli by serotyping.
The obtained results indicated that the coliform counts in the examined samples were varied from 2.7x102 to 9.1x104 with an average value of1.65x104+3.75x103 CFU/g for chicken thigh and 2.3x102 to 6.3x104 with an average value of 9.73x103+2.58x103 CFU/g for chicken breast respectively.
Coliform isolates were identified as E.coli, Citrobacter freundii, Citrobacter diversus, Enterbacter agglomerans, Enterbacter aerogenes, Enterbacter cloacae, Hafni alvie, serratia lqufaciens, and klebsiella pneumoniae subsp. ( pneumoniae and K. ozaenae).
Moreover, the incidence of serologically identified E. coli as Enteropathogenic E. coli (E coli O86:k61, and E coli O55:k59),Enterotoxogenic E. coli (E coli O125:k70, and E coli O128:k67)and Enterheamorrhagic E. coli (E coli O26:k60 and E coli O111:k58).
Antimicrobial resistance was carried out to 8 of isolated coliforms strains (E.coli, Citrobacter freundii, Enterbacter agglomerans, Enterbacter aerogenes Enterbacter cloacae, klebsiella pneumonia subsp. pneumonia, klebsiella pneumonia subsp. ozaenae and serratia liqufaciens), by detecting susceptibility to 8 antibiotics which was tested by disc diffusion. Generally, E.coli was resistant to ciprofloxacin, vancomycin, norfloxacin, streptomycin and kanamycin , Citrobacter freundii was resistant to ciprofloxacin, vancomycin, Erthromycin, and kanamycin , Enterbacter agglomerans was resistant to vancomycin, Erthromycin, cefoxitim, streptomycin, and kanamycin as antimicrobial agents. While, Enterbacter aerogenes was resistance to vancomycin, cefoxitim, and kanamycin. Also,Enterbacter cloacae and klebsiella pneumonia subsp. Ozaenae strains was resistance to ciprofloxacin, norfloxacin and kanamycin however, serratia liqufaciens strain was resistance to ciprofloxacin, Vancomycin, ,Cefoxitim , Norfloxacin and kanamycin .but klebsiella pneumonia subsp. pneumonia strain was sensitive to all tested antibiotic disc Doxyclin, ciprofloxacin, Vancomycin, Erthromycin, Cefoxitim, Norfloxacin, streptomycin, and kanamycin.
Garlic essential oils at concentration 0.5% the coliforms count was 6.57+4.76 log CFU/g. at 6th day compared with control sample6.76+4.76 log CFU/g. While at concentration1,5%of garlic essential oils, the results showed that coliforms counts was5.72+3.76 log CFU/g. Also thyme essential oils at concentration 0.5% had growth inhibition effect to coliform count to6.43+4.76 log CFU/g at the 6th day. And at concentration 1,5 %, the results showed that count of coliforms was5.40+3.7 log CFU/g . The result showed that thyme more effective than garlic essential oil in reduction of coliforms count.
from the above mentioned results revealed that the high count of coliforms this study might be attributed to absence of hygienic measures, excessive handling, ignorance of workers and vendors with hygienic measures and also using contaminated equipment in addition to lack of maintaining cold chain due to bad hygienic measures in many supermarkets.
It was also clear from experimental work that both thyme and garlic EOs showed significant antibacterial activity against coliforms in poultry meat samples and the inhibition of coliforms is related to the concentration of the studied essential oils.