الفهرس | Only 14 pages are availabe for public view |
Abstract Newly born calves represent an important source of animal production for either meat or breeding, diarrhea is one of the very common disease syndromes in neonatal calves, and it is causing more than half of all mortality in calves. E.coli is an important cause of diarrhea in farm animals. E.coli bacteria are a normal inhabitant in the intestine which can cause disease under stress conditions through production of enterotoxins. By bacteriological examination of (150) faecal samples collected from neonatal calves found that 55.33% of examined samples were positive for E.coli bacteria. fourteen randomly chosen E.coli isolates Serotyping resulted in 6 different serogroups O146(7.14%), O126(14.28%), O125(28.57%), O44(7.14%), O18(7.14%) and O6(7.14%) in addition to rough and untypeable strains(28.57%). Eight E.coli strains were tested by uniplex and multiplex PCR for characterization of 6 virulence genes (stx1, stx2, fimH, iutA, eaeA and tsh) resulted in (fimH) gene appear in all tested strains by percentage of 100% followed by 50% for iutA gene and 37.5% for eaeA and tsh genes while stx1, stx2 genes were not detected in any strain. |