الفهرس 
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Abstract
Objectives: To estimate the occurrence of Shiga toxin-producing E. coli (STEC) in cattle as their contact keepers Design: Descriptive study. Sampling: The study comprised 430 samples consisting of (n = 90) form cattle feces and milk samples (n = 90) alongside minced meat samples (n= 70) and stool specimens (n= 180) were collected from human contacts Procedures: All samples were examined using conventional bacteriological and molecular techniques. Results: The overall occurrence of E. coli in all collected samples was 48.83% in which 48.88%, 27.77%, 31.42% and 66% were isolated from cattle feces, milk, minced meat and human stool, respectively.. The most prevalent serotypes obtained from cattle and their keepers were O157, O128, O146, O113, O121, O91, O84, O86, O17, O11, O119, O111 and O26 and O55. The PCR analysis showed that stx1 was the most predominant genotypes followed by stx2. E .coli isolates showed high resistance to penicillin G (91.8%) followed by erythromycin and cephalothin (78.68%), ampicillin (72.13%). However, some isolates showed low resistance to ciprofloxacin (8.19%) followed by amikacin (6.55%) and finally gentamicin (3.27%). Whereas, 96.72%, 93.44%, 91.8%, 78.68%, 75.4% and 67.2% of tested E. coli isolates showed susceptibility to gentamicin, amikacin, ciprofloxacin, doxycycline, kanamycin and sulphamethoxazol, respectively. Conclusion: the presence of Shiga toxigenic and multiple drug-resistant strains of E. coli in the study area pose a high potential risk. Hence, strict hygienic measures should be followed to reduce the risk of STEC occurrence in cattle.