الفهرس 
Anatomical Aspect of the StomachOnly 14 pages are availabe for public view
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Abstract
Fifty adult male albino rats were used in the present study to evaluate the possible role of platelet rich plasma (PRP) on experimentally induced gastric ulcer by acetyle salicylic acid. Fourteen were occupied for preparation of PRP, the remaining (thirty six) were divided into five main groups:
group I (Control group): They were further subdivided into:
- Subgroup Ia (Plain control): consisted of six rats. They were kept without any treatment and served as control for all experimental groups.
- Subgroup Ib (sham control): consisted of six rats. Each received 0.6 ml of 1% carboxy methyl cellulose (the solvent used to dissolve acetyl salicylic acid) orally by gastric tube.
group II (PRP treated group): included six rats they received 0.5ml/kg body weight of PRP 2 days per week for 3 weeks subcutaneously.
group III (Aspirin induced gastric ulcer group): included six rats. Each rat received a single dose of aspirin (300 mg/kg body weight) dissolved in 0.6 ml of 1% carboxy methyl cellulose orally by gastric tube.
group IV (Aspirin induced gastric ulcer pretreated with PRP group): consisted of six rats. Each rat received a dose of 0.5ml/kg body weight of PRP, 2 days per week for3 weeks. One hour after administration of the last dose of PRP, a single oral dose of aspirin (300 mg/kg body weight) was administrated orally by gastric tube. Five hours later rats were sacrificed.
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group V (Aspirin induced gastric ulcer post-treated with PRP group): included six rats. Each rat received a single oral dose of aspirin (300 mg/kg body weight) orally by gastric tube. Five hours later each rat received a dose of 0.5ml/kg body weight of PRP 2 days per week for 3 weeks. One hour after administration of the last dose of PRP rats were sacrificed
The abdominal wall was opened exposing the stomach. The glandular stomach was excised, opened through the greater curvature part washed by saline to undergo histological and immunohistochemical studies.
A) Part of the specimens were collected, fixed in 10% neutral buffered formalin and processed for paraffin blocks formation 4-6 um thick sections for histological, immunohistochemical and morphometric studies.
1. Histological studies: Paraffin blocks were stained by the following stains: hematoxylin and eosin, PAS& Alician blue and Mallory trichrome.
2. Immunohistochemical studies: for detection of inducible nitric oxide synthtase (iNOS) and epidermal growth factor (EGF).
3. Morphometric measurements by the image analyzer for calculating percentage of surface area of PAS, collagen fiber deposition, iNOS and EGF immunoreactivity then statistical analysis were done.
B) The other part was fixed in 2.5% gluteraldehyde in phosphate buffer (0.1 mol/l) for scanning electron microscopic study.
Results: group I (control group) and group II (PRP group) showed similar histological results like normal stomach. group III (aspirin induced gastric ulcer group) showed extensive damage with loss of normal architecture of the fundus of the stomach. Most of the
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sections of the fundus showed excessive mononuclear cellular infilteration in the lamina propria and bases of the glands resulting in erosion, ulceration of the mucosa with separation and exfoliation of the surface epithelial cells. Other sections showed extravasation of blood with congestion of the blood vessels in the submucosa. PAS & alician blue stain, revealed absent PAS positive reaction in the superficial cells but cells in the bases contained PAS positive mucous also mucous neck cells contained AB (Alician blue) positive mucous. Mallory trichrome stain, showed normal amount of collagen fiber around dilated congested blood vessels in the submucosa but the amount of collagenous fibers was greatly increased in subgroup V and V.
Immunohistochemical study, revealed strong positive immunoreactivity of iNOS but weak positive EGF immunoreactivity. Groups IV and V showed strong positive EGF immunoreactivity.
All the previous findings were improved in group IV and V. This improvement was obvious in group IV more than group V.