الفهرس 
Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
This study was conducted to investigate the possible effects of vitamin D injection and its mechanisms of action on the progression of high fat diet (HFD) induced non- alcoholic fatty liver disease (NAFLD).
To do so, thirty adult male albino rats weighing 250-300grams were categorized into three equal groups.
1- Control group which was further divided into two subgroups; non-treated control group: rats were fed standard chow diet and tap water and vitamin D treated control group: rats were fed standard chow diet and tap water in addition to intra-peritoneal injection of vitamin D (5µg/kg body weight/every two days) for 12 weeks.
2- HFD group: rats were fed high fat diet (40% of total content of the diet was fat) and tap water for 12 weeks.
3- HFD group treated with vitamin D: rats were fed high fat diet (40% of total content of the diet was fat) and tap water in addition to intra-peritoneal injection of vitamin D (5 µg/kg body weight/every two days) for 12 weeks.
At the end of experiment duration, rats were decapitated, and blood samples were withdrawn from internal jugular vein for measurement of serum liver enzymes, serum free fatty acids (FFA) and serum tumor necrosis factor-α (TNF-α). Livers were dissected for histo-pathological examination and for measurement of tissue B- cell leukemia/lymphoma protein (BCL2), tissue calreticulin (CRT) and tissue calcium (Ca+2).
The results obtained showed that HFD fed rats had significant increase in mean body weight, mean liver weight and histopathological changes in the liver in the
form of moderate to severe ballooning of cells, micro and macro- vesicular steatosis, piece meal necrosis, inflammatory foci and periportal fibrosis along with significant increase in serum level of ALT, AST, FFA, TNF-α, liver’s CRT and ionized Calcium with significant decrease in liver’s BCL2 level when compared with the control group.
HFD rats treated with vitamin D had milder histopathological findings in the form of mild ballooning and mild steatosis in addition to significant decrease in serum ALT, AST, FFA, TNF-α, liver’s CRT and Ca+2 levels with significant increase in liver’s BCL2 level when compared with HFD non treated group.